PSXIII-B-8 Effect of Supplemental myo-Inositol on Growth Performance and Apparent Total Tract Digestibility of Weanling Piglets fed Reduced Protein Diets and Intestinal Cell Epithelial Barrier Integrity

Abstract Myo-inositol is a breakdown product of phytate produced in the gut through action phytase. Although effect phytase-released phosphorus on growth performance animals has been well characterized, there still little understanding myo-inositol effects. The objectives this study were to determine effects added phytate-rich low-protein diet and apparent total tract digestibility (ATTD) growing piglets as whether could directly affect intestinal epithelial cell proliferation function porcine cells (IPEC-J2). A 128 weanling allotted four dietary treatments consisting eight replicates per treatment replicate randomized complete block design for weeks. experimental diets comprised positive control (PC; 20% crude protein (CP), negative (NC; 17% CP), plus 2.0g/kg (NC+INO; CP) 3000FTU/kg phytase (NC+PHY; CP). Weekly average daily feed intake (ADFI), gain (ADG), gain-feed ratio (G: F) recorded. Phytase supplementation protein-deficient NC increased G:F (P< 0.05) without performance. Phosphorus supplemented group compared PC, NC, NC+INO groups whereas plasma concentration was significantly higher group. Due lack performance, an additional vitro conducted direct epithelium that might not be reflected mRNA abundance selected nutrient transporters concentration-dependent manner 0.05), enhanced barrier integrity IPEC-J2 monolayer reduced paracellular permeability FITC-dextran 0.05). In conclusion, despite animal in-vitro data indicates may regulate integrity.